Feil Family Brain & Mind Research Institute

You are here

rahu is a mutant allele of Dnmt3c, encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline.

Titlerahu is a mutant allele of Dnmt3c, encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline.
Publication TypeJournal Article
Year of Publication2017
AuthorsJain D, Meydan C, Lange J, Bouuaert CClaeys, Lailler N, Mason CE, Anderson KV, Keeney S
JournalPLoS Genet
Volume13
Issue8
Paginatione1006964
Date Published2017 Aug
ISSN1553-7404
KeywordsAlleles, Amino Acid Sequence, Animals, Chromosome Mapping, DNA (Cytosine-5-)-Methyltransferases, DNA Breaks, Double-Stranded, DNA Methylation, DNA Repair, Epigenetic Repression, Germ Cells, Infertility, Male, Male, Meiosis, Mice, Mice, Inbred C57BL, Mutation, Phylogeny, Protein Conformation, Retroelements, Sequence Analysis, RNA, Up-Regulation
Abstract

Transcriptional silencing by heritable cytosine-5 methylation is an ancient strategy to repress transposable elements. It was previously thought that mammals possess four DNA methyltransferase paralogs-Dnmt1, Dnmt3a, Dnmt3b and Dnmt3l-that establish and maintain cytosine-5 methylation. Here we identify a fifth paralog, Dnmt3c, that is essential for retrotransposon methylation and repression in the mouse male germline. From a phenotype-based forward genetics screen, we isolated a mutant mouse called 'rahu', which displays severe defects in double-strand-break repair and homologous chromosome synapsis during male meiosis, resulting in sterility. rahu is an allele of a transcription unit (Gm14490, renamed Dnmt3c) that was previously mis-annotated as a Dnmt3-family pseudogene. Dnmt3c encodes a cytosine methyltransferase homolog, and Dnmt3crahu mutants harbor a non-synonymous mutation of a conserved residue within one of its cytosine methyltransferase motifs, similar to a mutation in human DNMT3B observed in patients with immunodeficiency, centromeric instability and facial anomalies syndrome. The rahu mutation lies at a potential dimerization interface and near the potential DNA binding interface, suggesting that it compromises protein-protein and/or protein-DNA interactions required for normal DNMT3C function. Dnmt3crahu mutant males fail to establish normal methylation within LINE and LTR retrotransposon sequences in the germline and accumulate higher levels of transposon-derived transcripts and proteins, particularly from distinct L1 and ERVK retrotransposon families. Phylogenetic analysis indicates that Dnmt3c arose during rodent evolution by tandem duplication of Dnmt3b, after the divergence of the Dipodoidea and Muroidea superfamilies. These findings provide insight into the evolutionary dynamics and functional specialization of the transposon suppression machinery critical for mammalian sexual reproduction and epigenetic regulation.

DOI10.1371/journal.pgen.1006964
Alternate JournalPLoS Genet.
PubMed ID28854222
PubMed Central IDPMC5607212
Grant ListP30 CA008748 / CA / NCI NIH HHS / United States
R37 HD035455 / HD / NICHD NIH HHS / United States