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Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria.

TitlePlate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria.
Publication TypeJournal Article
Year of Publication2018
AuthorsWong H-S, Monternier P-A, Orr AL, Brand MD
JournalMethods Mol Biol
Volume1782
Pagination287-299
Date Published2018
ISSN1940-6029
Abstract

Superoxide and hydrogen peroxide produced by mitochondria play important roles in various physiological and pathological processes. This chapter describes a plate-based method to measure rates of superoxide and/or hydrogen peroxide production at specific sites in isolated mitochondria.

DOI10.1007/978-1-4939-7831-1_16
Alternate JournalMethods Mol. Biol.
PubMed ID29851006