Neuronal wiring diagram of an adult brain.

TitleNeuronal wiring diagram of an adult brain.
Publication TypeJournal Article
Year of Publication2023
AuthorsDorkenwald S, Matsliah A, Sterling AR, Schlegel P, Yu S-C, McKellar CE, Lin A, Costa M, Eichler K, Yin Y, Silversmith W, Schneider-Mizell C, Jordan CS, Brittain D, Halageri A, Kuehner K, Ogedengbe O, Morey R, Gager J, Kruk K, Perlman E, Yang R, Deutsch D, Bland D, Sorek M, Lu R, Macrina T, Lee K, J Bae A, Mu S, Nehoran B, Mitchell E, Popovych S, Wu J, Jia Z, Castro M, Kemnitz N, Ih D, Bates AShakeel, Eckstein N, Funke J, Collman F, Bock DD, Jefferis GSXE, H Seung S, Murthy M
Corporate AuthorsFlyWire Consortium
Date Published2023 Jul 11

Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative, yet inadequate for understanding brain function more globally. Here, we present the first neuronal wiring diagram of a whole adult brain, containing 5×107 chemical synapses between ~130,000 neurons reconstructed from a female Drosophila melanogaster. The resource also incorporates annotations of cell classes and types, nerves, hemilineages, and predictions of neurotransmitter identities. Data products are available by download, programmatic access, and interactive browsing and made interoperable with other fly data resources. We show how to derive a projectome, a map of projections between regions, from the connectome. We demonstrate the tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine, and descending neurons), across both hemispheres, and between the central brain and the optic lobes. Tracing from a subset of photoreceptors all the way to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviors. The technologies and open ecosystem of the FlyWire Consortium set the stage for future large-scale connectome projects in other species.

Alternate JournalbioRxiv
PubMed ID37425937
PubMed Central IDPMC10327113
Grant ListRF1 NS121911 / NS / NINDS NIH HHS / United States
/ WT_ / Wellcome Trust / United Kingdom
U24 NS126935 / NS / NINDS NIH HHS / United States
RF1 MH129268 / MH / NIMH NIH HHS / United States
RF1 MH120679 / MH / NIMH NIH HHS / United States
RF1 MH117815 / MH / NIMH NIH HHS / United States