|Modeling the Spatiotemporal Dynamics of Light and Heat Propagation for In Vivo Optogenetics.
|Year of Publication
|Stujenske JM, Spellman T, Gordon JA
|2015 Jul 21
|Animals, Brain, Hot Temperature, Light, Mice, Models, Theoretical, Neurons, Optogenetics, Spatio-Temporal Analysis
Despite the increasing use of optogenetics in vivo, the effects of direct light exposure to brain tissue are understudied. Of particular concern is the potential for heat induced by prolonged optical stimulation. We demonstrate that high-intensity light, delivered through an optical fiber, is capable of elevating firing rate locally, even in the absence of opsin expression. Predicting the severity and spatial extent of any temperature increase during optogenetic stimulation is therefore of considerable importance. Here, we describe a realistic model that simulates light and heat propagation during optogenetic experiments. We validated the model by comparing predicted and measured temperature changes in vivo. We further demonstrate the utility of this model by comparing predictions for various wavelengths of light and fiber sizes, as well as testing methods for reducing heat effects on neural targets in vivo.
|PubMed Central ID
|R01 MH081968 / MH / NIMH NIH HHS / United States
R01 MH096274 / MH / NIMH NIH HHS / United States