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Modeling the Spatiotemporal Dynamics of Light and Heat Propagation for In Vivo Optogenetics.

TitleModeling the Spatiotemporal Dynamics of Light and Heat Propagation for In Vivo Optogenetics.
Publication TypeJournal Article
Year of Publication2015
AuthorsStujenske JM, Spellman T, Gordon JA
JournalCell Rep
Volume12
Issue3
Pagination525-34
Date Published2015 Jul 21
ISSN2211-1247
KeywordsAnimals, Brain, Hot Temperature, Light, Mice, Models, Theoretical, Neurons, Optogenetics, Spatio-Temporal Analysis
Abstract

Despite the increasing use of optogenetics in vivo, the effects of direct light exposure to brain tissue are understudied. Of particular concern is the potential for heat induced by prolonged optical stimulation. We demonstrate that high-intensity light, delivered through an optical fiber, is capable of elevating firing rate locally, even in the absence of opsin expression. Predicting the severity and spatial extent of any temperature increase during optogenetic stimulation is therefore of considerable importance. Here, we describe a realistic model that simulates light and heat propagation during optogenetic experiments. We validated the model by comparing predicted and measured temperature changes in vivo. We further demonstrate the utility of this model by comparing predictions for various wavelengths of light and fiber sizes, as well as testing methods for reducing heat effects on neural targets in vivo.

DOI10.1016/j.celrep.2015.06.036
Alternate JournalCell Rep
PubMed ID26166563
PubMed Central IDPMC4512881
Grant ListR01 MH081968 / MH / NIMH NIH HHS / United States
R01 MH096274 / MH / NIMH NIH HHS / United States