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The Impact of Heterogeneity on Single-Cell Sequencing.

TitleThe Impact of Heterogeneity on Single-Cell Sequencing.
Publication TypeJournal Article
Year of Publication2019
AuthorsGoldman SL, MacKay M, Afshinnekoo E, Melnick AM, Wu S, Mason CE
JournalFront Genet
Volume10
Pagination8
Date Published2019
ISSN1664-8021
Abstract

The importance of diversity and cellular specialization is clear for many reasons, from population-level diversification, to improved resiliency to unforeseen stresses, to unique functions within metazoan organisms during development and differentiation. However, the level of cellular heterogeneity is just now becoming clear through the integration of genome-wide analyses and more cost effective Next Generation Sequencing (NGS). With easy access to single-cell NGS (scNGS), new opportunities exist to examine different levels of gene expression and somatic mutational heterogeneity, but these assays can generate yottabyte scale data. Here, we model the importance of heterogeneity for large-scale analysis of scNGS data, with a focus on the utilization in oncology and other diseases, providing a guide to aid in sample size and experimental design.

DOI10.3389/fgene.2019.00008
Alternate JournalFront Genet
PubMed ID30881372
PubMed Central IDPMC6405636
Grant ListR01 MH117406 / MH / NIMH NIH HHS / United States
R01 AI125416 / AI / NIAID NIH HHS / United States
R01 ES021006 / ES / NIEHS NIH HHS / United States
R25 EB020393 / EB / NIBIB NIH HHS / United States
R21 AI129851 / AI / NIAID NIH HHS / United States
R01 NS076465 / NS / NINDS NIH HHS / United States