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How many molecules of mitochondrial complex I are in a cell?

TitleHow many molecules of mitochondrial complex I are in a cell?
Publication TypeJournal Article
Year of Publication2022
AuthorsAnsari F, Yoval B, Niatsetskaya Z, Ten V, Wittig I, Galkin A
JournalAnal Biochem
Pagination114646
Date Published2022 Mar 05
ISSN1096-0309
Abstract

Mitochondrial complex I is the only enzyme responsible for oxidation of matrix NADH and regeneration of NAD+ for catabolism. Nuclear and mtDNA mutations, assembly impairments, and enzyme damage are implicated in inherited diseases, ischemia-reperfusion injury, neurodegeneration, and tumorogenesis. Here we introduce a novel method to measure the absolute content of complex I. The method is based on flavin fluorescence scanning of a polyacrylamide gel after separation of complexes by Clear Native electrophoresis. Using mouse primary astrocytes as an example, we calculated an average value of 2.2 × 105 complex I molecules/cell. Our method can be used for accurate quantification of complex I content.

DOI10.1016/j.ab.2022.114646
Alternate JournalAnal Biochem
PubMed ID35259403