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Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius.

TitleGenome assembly and geospatial phylogenomics of the bed bug Cimex lectularius.
Publication TypeJournal Article
Year of Publication2016
AuthorsRosenfeld JA, Reeves D, Brugler MR, Narechania A, Simon S, Durrett R, Foox J, Shianna K, Schatz MC, Gandara J, Afshinnekoo E, Lam ET, Hastie AR, Chan S, Cao H, Saghbini M, Kentsis A, Planet PJ, Kholodovych V, Tessler M, Baker R, DeSalle R, Sorkin LN, Kolokotronis S-O, Siddall ME, Amato G, Mason CE
JournalNat Commun
Volume7
Pagination10164
Date Published2016 Feb 02
ISSN2041-1723
KeywordsAnimals, Bedbugs, Blood, Chromosome Mapping, Eating, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, Humans, Life Cycle Stages, Male, Models, Molecular, Phylogeny, Sequence Analysis, RNA
Abstract

The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

DOI10.1038/ncomms10164
Alternate JournalNat Commun
PubMed ID26836631
PubMed Central IDPMC4740774
Grant ListF31 GM111053 / GM / NIGMS NIH HHS / United States
R01NS076465 / NS / NINDS NIH HHS / United States
P30 CA008748 / CA / NCI NIH HHS / United States
T32 GM083937 / GM / NIGMS NIH HHS / United States
F31GM111053 / GM / NIGMS NIH HHS / United States
R01 GM092706 / GM / NIGMS NIH HHS / United States
R01 NS076465 / NS / NINDS NIH HHS / United States
1T32GM083937 / GM / NIGMS NIH HHS / United States
R01GM092706 / GM / NIGMS NIH HHS / United States