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Estrogen receptor α and G-protein coupled estrogen receptor 1 are localized to GABAergic neurons in the dorsal striatum.

TitleEstrogen receptor α and G-protein coupled estrogen receptor 1 are localized to GABAergic neurons in the dorsal striatum.
Publication TypeJournal Article
Year of Publication2016
AuthorsAlmey A, Milner TA, Brake WG
JournalNeurosci Lett
Volume622
Pagination118-23
Date Published2016 05 27
ISSN1872-7972
KeywordsAnimals, Corpus Striatum, Estrogen Receptor alpha, Female, GABAergic Neurons, Rats, Sprague-Dawley, Receptors, G-Protein-Coupled
Abstract

Estrogens affect dopamine transmission in the striatum, increasing dopamine availability, maintaining D2 receptor density, and reducing the availability of the dopamine transporter. Some of these effects of estrogens are rapid, suggesting that they are mediated by membrane associated receptors. Recently our group demonstrated that there is extra-nuclear labeling for ERα, ERβ, and GPER1 in the striatum, but that ERα and GPER1 are not localized to dopaminergic neurons in this region. GABAergic neurons are the most common type of neuron in the striatum, and changes in GABA transmission affect dopamine transmission. Thus, to determine whether ERα or GPER1 are localized to GABAergic neurons, we double labeled the striatum with antibodies for ERα or GPER1 and GABA and examined them using electron microscopy. Ultrastructural analysis revealed that ERα and GPER1 are localized exclusively to extranuclear sites in the striatum, and ∼35% of the dendrites and axon terminals labeled for these receptors contain GABA immunoreactivity. Binding at membrane-associated ERα and GPER1 could account for rapid estrogen-induced decreases in GABA transmission in the striatum, which, in turn, could affect dopamine transmission in this region.

DOI10.1016/j.neulet.2016.04.023
Alternate JournalNeurosci. Lett.
PubMed ID27080432
PubMed Central IDPMC5104174
Grant ListP01 AG016765 / AG / NIA NIH HHS / United States
P01 HL096571 / HL / NHLBI NIH HHS / United States
R01 DA008259 / DA / NIDA NIH HHS / United States
R01 HL098351 / HL / NHLBI NIH HHS / United States