We are seeing patients in-person and through Video Visits. Learn more about how we’re keeping you safe and please review our updated visitor policy. Please also consider supporting Weill Cornell Medicine’s efforts to support our front-line workers.
Feil Family Brain & Mind Research Institute

You are here

Distribution and localization of phosphatidylinositol 5-phosphate, 4-kinase alpha and beta in the brain.

TitleDistribution and localization of phosphatidylinositol 5-phosphate, 4-kinase alpha and beta in the brain.
Publication TypeJournal Article
Year of Publication2020
AuthorsNoch EK, Yim I, Milner TA, Cantley LC
JournalJ Comp Neurol
Date Published2020 May 24
ISSN1096-9861
Abstract

Phosphatidylinositol-4,5-bisphosphate (PI-4,5-P ) is critical for synaptic vesicle docking and fusion and generation of the second messengers, diacylglycerol and inositol-1,4,5-trisphosphate. PI-4,5-P can be generated by two families of kinases: type 1 phosphatidylinositol-4-phosphate 5-kinases, encoded by PIP5K1A, PIP5K1B and PIP5K1C, and type 2 phosphatidylinositol-5-phosphate 4-kinases, encoded by PIP4K2A, PIP4K2B, and PIP4K2C. While the roles of the type 1 enzymes in brain function have been extensively studied, the roles of the type 2 enzymes are poorly understood. Using selective antibodies validated by genetic deletion of pip4k2a or pip4k2b in mouse brain, we characterized the location of the enzymes, PI5P4Kα and PI5P4Kß, encoded by these genes. In mice, we demonstrate that PI5P4Kα is expressed in adulthood, whereas PI5P4Kß is expressed early in development. PI5P4Kα localizes to white matter tracts, especially the corpus callosum, and at a low level in neurons, while PI5P4Kß is expressed in neuronal populations, especially hippocampus and cortex. Dual labeling studies demonstrate that PI5P4Kα co-localizes with the oligodendrocyte marker, Olig2, whereas PI5P4Kß co-localizes with the neuronal marker, NeuN. Ultrastructural analysis demonstrates that both kinases are contained in axon terminals and dendritic spines adjacent to the synaptic membrane, which support a potential role in synaptic transmission. Immunoperoxidase analysis of macaque and human brain tissue demonstrate a conserved pattern for PI5P4Kα and PI5P4Kß. These results highlight the diverse cell-autonomous expression of PI5P4Kα and PI5P4Kß and support further exploration into their role in synaptic function in the brain.

DOI10.1002/cne.24956
Alternate JournalJ. Comp. Neurol.
PubMed ID32449185
Grant ListR01 HL136520 / HL / NHLBI NIH HHS / United States
R35 CA197588 / CA / NCI NIH HHS / United States