Feil Family Brain & Mind Research Institute

You are here

Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection.

TitleDetermination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection.
Publication TypeJournal Article
Year of Publication2017
AuthorsShurubor YI, D'Aurelio M, Clark-Matott J, Isakova EP, Deryabina YI, M Beal F, Cooper AJL, Krasnikov BF
JournalMolecules
Volume22
Issue9
Date Published2017 Aug 23
ISSN1420-3049
Abstract

Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, mouse cortex, and rat plasma, liver, kidney, and brain tissues. The limits of detection for CoA and acetyl-CoA are >10-fold lower than those obtained by previously described HPLC procedures, with coefficients of variation <1% for standard solutions, and 1-3% for deproteinized biological samples. Recovery is 95-97% for liver extracts spiked with Co-A and acetyl-CoA. Many factors may influence the tissue concentrations of CoA and acetyl-CoA (e.g., age, fed, or fasted state). Nevertheless, the values obtained by the present HPLC method for the concentration of CoA and acetyl-CoA in selected rodent tissues are in reasonable agreement with literature values. The concentrations of CoA and acetyl-CoA were found to be very low in rat plasma, but easily measurable by the present HPLC method. The method should be useful for studying cellular energy metabolism under normal and pathological conditions, and during targeted drug therapy treatment.

DOI10.3390/molecules22091388
Alternate JournalMolecules
PubMed ID28832533