Cell-type-specific inhibitory circuitry from a connectomic census of mouse visual cortex.

TitleCell-type-specific inhibitory circuitry from a connectomic census of mouse visual cortex.
Publication TypeJournal Article
Year of Publication2023
AuthorsSchneider-Mizell CM, Bodor A, Brittain D, Buchanan JA, Bumbarger DJ, Elabbady L, Kapner D, Kinn S, Mahalingam G, Seshamani S, Suckow S, Takeno M, Torres R, Yin W, Dorkenwald S, J Bae A, Castro MA, Fahey PG, Froudakis E, Halageri A, Jia Z, Jordan C, Kemnitz N, Lee K, Li K, Lu R, Macrina T, Mitchell E, Mondal SSubhra, Mu S, Nehoran B, Papadopoulos S, Patel S, Pitkow X, Popovych S, Silversmith W, Sinz FH, Turner NL, Wong W, Wu J, Yu S-C, Reimer J, Tolias AS, H Seung S, R Reid C, Collman F, da Costa NMa├žarico
Corporate AuthorsMICrONS Consortium
JournalbioRxiv
Date Published2023 Feb 14
Abstract

Mammalian cortex features a large diversity of neuronal cell types, each with characteristic anatomical, molecular and functional properties. Synaptic connectivity rules powerfully shape how each cell type participates in the cortical circuit, but comprehensively mapping connectivity at the resolution of distinct cell types remains difficult. Here, we used millimeter-scale volumetric electron microscopy to investigate the connectivity of inhibitory neurons across a dense neuronal population spanning all layers of mouse visual cortex with synaptic resolution. We classified all 1183 excitatory neurons within a 100 micron column into anatomical subclasses using quantitative morphological and synapse features based on full dendritic reconstructions, finding both familiar subclasses corresponding to axonal projections and novel intralaminar distinctions based on synaptic properties. To relate these subclasses to single-cell connectivity, we reconstructed all 164 inhibitory interneurons in the same column, producing a wiring diagram of inhibition with more than 70,000 synapses. We found widespread cell-type-specific inhibition, including interneurons selectively targeting certain excitatory subpopulations among spatially intermingled neurons in layer 2/3, layer 5, and layer 6. Globally, inhibitory connectivity was organized into "motif groups," heterogeneous collections of cells that collectively target both perisomatic and dendritic compartments of the same combinations of excitatory subtypes. We also discovered a novel category of disinhibitory-specialist interneuron that preferentially targets basket cells. Collectively, our analysis revealed new organizing principles for cortical inhibition and will serve as a powerful foundation for linking modern multimodal neuronal atlases with the cortical wiring diagram.

DOI10.1101/2023.01.23.525290
Alternate JournalbioRxiv
PubMed ID36747710
PubMed Central IDPMC9900837